Superoxide Dismutase from bovine liver 

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO.¹ SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.^2,3

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

Lyophilized powder containing potassium phosphate buffer salts

lyophilized powder

Protein, 70-90% biuret

cow ... SOD1(281495)

−20°C

1. Beckman, J.S., et al., Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc. Natl. Acad. Sci. USA 87, 1620-1624, (1990)

2. Tilly, J.L., and Tilly, K.I., Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. Endocrinology 136, 242-252, (1995)

3. Keller, J.N., et al., Mitochondrial manganese superoxide dismutase prevents neural apoptosis and reduces ischemic brain injury. J. Neurosci. 18, 687-697, (1998)

Steinman, H.M., et al. J. Biol. Chem. 249, 7326, (1974) abstract

Merck 13,9092

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