SIRT1 human 

Solution containing 50 mM Tris, pH 7.4, 100 mM NaCl, 1 mM DTT, protease inhibitors (Product code P8340) 1:200, and 10% glycerol (w/v).

Sirtuins are a family of NAD⁺ dependent deacetylases that remove an acetyl group from the e-amino group of lysine residues. The proteins within this family are named after the first protein discovered, from yeast, called Sir2 (Silent Information Regulator 2). The proteins are conserved from bacteria to higher eukaryotes. In humans, there are seven Sir2 family members (SIRT1 to SITR7). SIRT1 plays a pivotal role in the regulation of cellular differentiation, metabolism, cell cycle, apoptosis and regulation of p53. Several targets for SIRT1 were identified among them Lys³⁸² of p53.¹ Using RNA interference, additional targets were identified. It was demonstrated that reduced levels of human SIRT1 led to increased acetylation of Histone H4-Lys¹⁶, H4-Lys²⁰, and Histone H3-Lys⁹ as well as histone H1-Lys²⁶.²

expressed in Escherichia coli

full-length amino acid sequence of original SIRT1 protein (accession number NP_036370)

>90% (SDS-PAGE)

buffered aqueous glycerol solution

human ... SIRT1(23411)

dry ice

−20°C

1. Vaziri, H., et al., hSIR2-SIRT1 functions as an NAD-dependent p53 deacetylase. Cell 107, 149-159, (2001)

2. Vaquero, A., et al., Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin. Mol. Cell. 16, 93-105, (2004)

CS1040, SIRT1 Assay Kit

S5196, Monoclonal Anti-Sirt1 antibody produced in mouse

S5322, Anti-Sirt1 (C-terminal) antibody produced in rabbit

S5447, Anti-SIRT1 antibody produced in rabbit