S8939
Sigma

SGK1 (δ1-60, S422D), active human

recombinant, expressed in Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged), buffered aqueous solution

MDL number

MFCD03458482

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Descriptions

Biochem/physiol Actions

Kinase assays.

Unit Definition

One unit of SGK1 = 1 nmol phosphate incorporated into crosstide substrate peptide in one minute.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, 0.15 M NaCl, 0.03% Brij-35, 1 mM benzamidine, 0.2 mM PMSF. and 270 mM sucrose.

Properties

recombinant

expressed in Sf9 cells (truncated human sequence (a.a. 1-60 deleted, S422D mutation)-histidine-tagged)

form

buffered aqueous solution

Gene Information

human ... SGK(6446)

shipped in

dry ice

storage temp.

−70°C

References

Reference

Kobayashi, T., Cohen, P., Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. Biochem. J. 339, 319, (1999)

Casamayor, A., Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr. Biol. 9, 186, (1999)

Safety

Safety Statements

23-24/25

WGK Germany