MISSION^® shRNA Human Gene Family Set, Bacterial Glycerol Stock 

The clones are sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) provided as frozen bacterial glycerol stocks (Terrific Broth, carbenicillin at 100 μg/ml and 10% glycerol). The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

For a detailed listing of other available gene family sets, visit the gene family set website.

Number of Genes: 112, Number of Clones: 615

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

human ... ARIH1(25820), ATE1(11101), ATXN3(4287), BAP1(8314), COPS5(10987), COPS6(10980), COPS8(10920), CYLD(1540), DUB3(377630), DUB4(391622), GPS1(2873), ITCH(83737), OTUB1(55611), OTUB2(78990), OTUD7B(56957), PSMA1(5682), PSMA2(5683), PSMA3(5684), PSMA4(5685), PSMA5(5686), PSMA6(5687), PSMA7(5688), PSMA8(143471), PSMB1(5689), PSMB10(5699), PSMB2(5690), PSMB3(5691), PSMB4(5692), PSMB5(5693), PSMB6(5694), PSMB7(5695), PSMB8(5696), PSMB9(5698), PSMC1(5700), PSMC2(5701), PSMC3(5702), PSMC4(5704), PSMC5(5705), PSMC6(5706), PSMD1(5707), PSMD10(5716), PSMD11(5717), PSMD12(5718), PSMD13(5719), PSMD14(10213), PSMD2(5708), PSMD3(5709), PSMD4(5710), PSMD5(5711), PSMD7(5713), PSMD8(5714), PSMD9(5715), PSME1(5720), PSME2(5721), PSME3(10197), PSMF1(9491), RAD23A(5886), RAD23B(5887), SHFM1(7979), SQSTM1(8878), STAMBP(10617), STAMBPL1(57559), UBASH3A(53347), UBE2O(63893), UBL5(59286), UBP1(7342), UBQLN1(29979), UBQLN2(29978), UBQLN3(50613), UBQLN4(56893), UBR1(197131), UBR2(23304), UCHL1(7345), UCHL3(7347), UCHL5(51377), UFD1L(7353), USP1(7398), USP10(9100), USP11(8237), USP12(219333), USP13(8975), USP14(9097), USP15(9958), USP16(10600), USP18(11274), USP19(10869), USP2(9099), USP20(10868), USP21(27005), USP22(23326), USP25(29761), USP26(83844), USP28(57646), USP29(57663), USP3(9960), USP30(84749), USP31(57478), USP32(84669), USP33(23032), USP34(9736), USP36(57602), USP37(57695), USP38(84640), USP39(10713), USP4(7375), USP40(55230), USP44(84101), USP46(64854), USP47(55031), USP48(84196), USP49(25862), USP5(8078), USP50(373509), USP51(158880), USP52(9924), USP53(54532), USP54(159195), USP6(9098), USP7(7874), USP8(9101), USP9X(8239), USP9Y(8287), VCP(7415), VPS11(55823)

−70°C

Millard SM, Wood SA, Riding the DUBway: regulation of protein trafficking by deubiquitylating enzymes J. Cell Biol. 173, 463-8 , (2006)

Nicholson B, et. al., Deubiquitinating enzymes as novel anticancer targets Future Oncol. 3, 191-9, (2007)