MISSION^® shRNA Human Gene Family Set, Lentiviral Particles 

Lentiviral particles are provided in 96-well plates that are barcoded for simple identification. Each gene family set is representationally titered (10% of clones). Fully titered sets are available on a custom basis. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.

For a detailed listing of other available gene family sets, visit the gene family set website.

Number of Genes: 46, Number of Clones: 209

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .

human ... AR(367), ESR1(2099), ESR2(2100), ESRRA(2101), ESRRB(2103), ESRRG(2104), HNF4A(3172), HNF4G(3174), NR0B1(190), NR0B2(8431), NR1D1(9572), NR1D2(9975), NR1H2(7376), NR1H3(10062), NR1H4(9971), NR1I2(8856), NR1I3(9970), NR2C1(7181), NR2C2(7182), NR2E1(7101), NR2E3(10002), NR2F2(7026), NR2F6(2063), NR3C2(4306), NR4A1(3164), NR4A2(4929), NR4A3(8013), NR5A1(2516), NR5A2(2494), NR6A1(2649), PGR(5241), PPARA(5465), PPARD(5467), PPARG(5468), RARA(5914), RARB(5915), RARG(5916), RORA(6095), RORB(6096), RORC(6097), RXRA(6256), RXRB(6257), RXRG(6258), THRA(7067), THRB(7068), VDR(7421)

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Burns, J. C., et al., Vesicular Stomatitis Virus G Glyocprotein Pseudotyped Retroviral Vectors: Concentration to a Very High Titer and Efficient Gene Transfer into Mammalian and Nonmammalian Cells Proc. Natl. Acad. Sci. USA 90, 8033-8037, (1993) abstract

Rosen, J., et al., Nuclear hormone receptor assays for drug discovery Drug Des. Discovery 2nd ed., 6, 224-230, (2003)

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Stewart, S. A., et al., Lentivirus-delivered stable gene silencing by RNAi in primary cells RNA 9, 493-501, (2003) abstract