MISSION^® TurboGFP^™ shRNA Control Vector 

To see more application data, protocols, vector maps visit sigma.com/shrna.

The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP.¹ The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.^2,3 The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Use of this product is subject to one or more license agreements. For details, please see the technical bulletin link.

TurboGFP is a trademark of Evrogen Co.

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

−20°C

1. Stewart, S.A., et al., Lentivirus-delivered stable gene silencing by RNAi in primary cells RNA 9, 493-501, (2003) abstract

2. Zufferey, R., et al., Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo Nat. Biotechnol. 15, 871-885, (1997)

3. Zufferery, R., et. al., Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery J. Virol. 72, 9873-9880, (1998) abstract

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