MISSION shRNA clones are sequence-verified shRNA lentiviral plasmids for gene silencing in mammalian cells (human or mouse). The parental vector (pLKO.1<-puro) allows for transient transfection or stable selection via puromycin resistance. In addition, the plasmids may be used to generate lentiviral transduction particles in packaging cell lines. 1 µg of plasmid DNA in TE buffer is provided for each clone and is ready for transfection or production of viral particles. Orders of 5 or less target sets (< 25 clones or constructs) are provided in individual vials. Orders of more than 5 target sets (> 25 clones or constructs) are provided in barcoded 96-well plates with a CD containing plate map positions.