Synaptic Vesicles Isolation Kit 

The Synaptic Vesicles Isolation Kit provides all the reagents required for preparing an enriched synaptic vesicle fraction. The isolation procedure involves tissue homogenization, hypo-osmotic lysis of the synaptosomes, and differential centrifugation followed by an OptiPrep density gradient. The kit also contains an antibody specific for synaptophysin, a synaptic vesicle marker, to follow the enrichment procedure. The kit was tested on rat and rabbit brain tissues.

−20°C

Homogenization Buffer 5X 125 mL

Lysis Buffer 5x 60 mL

Storage Buffer for Synaptic Vesicles 5X 30 mL

OptiPrep^® Density Gradient Medium 100 mL D1556

Monoclonal Anti-Synaptophysin antibody produced in mouse, clone SVP-38, ascites fluid 100 mL S5768