T0198
Sigma

Monoclonal Anti-β-Tubulin antibody produced in mouse

~1 mg/mL, clone D66, purified immunoglobulin, buffered aqueous solution

MDL number

MFCD03792414

Expand/Collapse All

Descriptions

Application

Can be used in studies involving the inhibition of flagellar motility in permeabilized sperm models.

Immunogen

sea urchin (Lytechinus pictus) sperm axonemal proteins.

Specificity

Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

D66

form

buffered aqueous solution

mol wt

antigen mol wt ~50 kDa

species reactivity

human, monkey, bovine, canine, rabbit, hamster, rat, mouse, chicken, sea urchin (Lytechinus pictus and Paracentrotus lividus), Chlamydomonas reinhardtii, Oxyrrhis marina (dinoflagellate)

concentration

~1 mg/mL

application(s)

immunoblotting: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells

immunocytochemistry: suitable

immunoprecipitation: suitable

indirect ELISA: suitable

isotype

IgG1

Gene Information

mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613)

rat ... Tubb2(29212), Tubb2c(296554)

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Audebert, S., et al. Eur. J. Biochem. 261, 48, (1999) abstract

Gingras, D., et al. Mol. Biol. Cell 9, 513, (1998) abstract

Safety

WGK Germany

Related Categories

... Stem Cell Characterization > Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology

... Antibodies to Cytoskeleton > Antibodies to Microtubule and Associated Proteins

... Clone Index > D

... Alphabetical Index > T

T0198 - Datasheet (33kb)

MSDS

Certificate of Analysis

More Information