Trichostatin A 

Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo.¹ Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription.^2,3 TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.⁴

from Streptomyces sp.

DMSO solution

5 mM in DMSO (0.2 μm-filtered)

dry ice

−20°C

1. Minucci, S., et al., A histone deacetylase inhibitor potentiates retinoid receptor action in embryonal carcinoma cells. Proc. Natl. Acad. Sci. USA 94, 11295-11300, (1997) abstract

2. Marks, P.A., et al., Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells. J. Natl. Cancer Inst. 92, 1210-1216, (2000)

3. Furumai, R., et al., Potent histone deacetylase inhibitors built from trichostatin A and cyclic tetrapeptide antibiotics including trapoxin. Proc. Natl. Acad. Sci. USA 98, 87-92, (2001)

4. Kishigami, S., et al., Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer. J. Reprod. Dev. 53, 165-170, (2007)

T8552, Trichostatin A