Trizma^® hydrochloride solution 

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Trizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

for molecular biology

0.2 μm filtered

1 M

DNase, RNase and protease, none detected

7.2

2