General description
The TargeTron Vector pACD4K-C-loxP is a 7,745bp E. coli expression vector for use with the TargeTron Gene Knockout System (TA0100). The vector is linearized at the HindIII and BsrGI sites. This vector differs from the original pACD4K-C vector (included in the TargeTron Gene Knockout System) in that it has loxP sites flanking each end of the kanamycin ORF that allow for removal of the kanamycin resistance marker via Cre-loxP mediated recombination. After removal of the kanamycin resistance marker, sequential knockouts can be generated.
Other Notes
Legal Information
TargeTron is a registered trademark of InGex, LLC.