3,3′,5,5′-Tetramethylbenzidine 

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine¹ suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,² assay of hemoglobin,^3,4,5 assay of peroxidases.^6,7,8

≥98% (TLC)

168-171 °C(lit.)

2-8°C

1. Holland, V.R., et al. Tet. 30, 3299, (1974)

2. D.D. Garner et al. J. Forensic Sci. 21, 819, (1976)

3. J.C. Standefer, D. Vanderjagt Clin. Chem. 23, 749, (1977)

4. R.C. Lijana, M.C. Williams J. Lab. Clin. Med. 94, 266, (1979)

5. Liem, H.H. et al. Anal. Biochem. 98, 388, (1979)

6. Bos, E.S., et al. J. Immunoassay 2, 187, (1981)

7. P.C. Andrews, N.I. Krinsky Anal. Biochem. 127, 346, (1982)

8. H. Gallati, I. Pracht J. Clin. Chem. Clin. Biochem. 23, 453, (1985)

Aldrich MSDS 1, 1679:B / Corp MSDS 1 (2), 3292:A / FT-IR 2 (2), 2090:C / FT-IR 1 (1), 1236:D / FT-NMR 1 (1), 1236:D / FT-NMR 1 (2), 529:B / IR-Spectra (3), 740:D / RegBook 1 (1), 1423:J / Sigma FT-IR 1 (2), 218:D / Stains and Dyes Ref, 693 / Structure Index 1, 222:D:3

Xi

36/37/38

26-36

DV2300000

8-10