Monoclonal Anti-Talin antibody produced in mouse 

purified chicken gizzard talin.

Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts.

ascites fluid

8d4

antigen mol wt 225-235 kDa

15 mM sodium azide

chicken, frog, human, mouse, rat

immunoblotting: suitable

indirect immunofluorescence: 1:500 using cultured Rat1 cells

IgG1

human ... TLN1(7094), TLN2(83660)

rat ... Tln1(313494)

mouse ... Tln1(21894), Tln2(70549)

dry ice

−20°C

Otey, C., et al., Characterization of monoclonal antibodies to chicken gizzard talin. Hybridoma 9, 57, (1990)

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