T3819
Sigma

Tetanus toxin C-fragment−FITC from Clostridium tetani

Synonym

TTC-FITC

MDL number

MFCD03458701

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Descriptions

General description

binding activity to GT1b gangliosides has been confirmed by the hemagglutination assay of Tayot, et al.

The μg amount of FITC bound per mg of C-fragment is determined assuming an extinction coefficient at 493 nm of 85,200 M^-1cm^-1 for FITC

Preparation Note

Prepared by conjugating the C-fragment of tetanus toxin to fluorescein isothiocyanate, isomer I (FITC) by a modification of the method of Wood et al.

Reconstitution

When reconstituted with 0.125 ml of sterile water, each vial contains 25 μg of protein in 0.01 m sodium phosphate, pH 7.5. Protect from light.

Biochem/physiol Actions

C terminal portion conjugated to fluorescein isothiocyanate is taken up by persynaptic nerve terminals and exhibits transsynaptic retrograde transport in central neurons similar to that of tetanus toxin. It is used to map neuronal connections and to target proteins to neurons

Properties

Gene Information

Clostridium tetani E88 ... tetX(1061100)

storage temp.

2-8°C

References

Reference

Wood, B. et al, Fluorescent antibody staining. 3. Preparation of fluorescein- isothiocyanate-labeled antibodies. J. Immunol. 95, 225, (1965) abstract

Tayot, J., et al, Receptor-specific large-scale purification of cholera toxin on silica beads derivatized with lysoGM1 ganglioside. Eur. J. Biochem. 113, 249, (1981) abstract

Onifer, S.M., et al. Cell. Transplant. 2, 131, (1993)

Safety

Hazard Codes

T+

Risk Statements

26/27/28

Safety Statements

WGK Germany

RTECS

XW5807000