T4393
Sigma

Thrombin from human plasma

lyophilized powder, 1500-3500 NIH units/mg protein (E1%/280, 18.3), cell culture tested

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Synonym

Factor IIa

CAS Number

9002-04-4

Enzyme Commission (EC) Number

3.4.21.5 ( BRENDA | IUBMB )

MDL number

MFCD00082072

EG/EC Number

232-648-7

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Descriptions

Analysis Note

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Reconstitution

When reconstituted with 1 mL water, vial contains stated activity in 0.15 M sodium chloride and 0.05 M sodium citrate, pH 6.5.

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Properties

form

lyophilized powder

total impurities

HIV and HBsAg, source material tested negative

solubility

H₂O: 1 mL/vial clear to slightly hazy, colorless solution

suitability

cell culture tested

Gene Information

human ... F2(2147)

storage temp.

−20°C

References

Reference

, R. Biggs, ed. Human Blood Coagulation, Haemostasis and Thrombosis 2nd ed., Philadelphia , (1976), 722

Merck

Merck 13,9461

Safety

Hazard Codes

Xn,B

Risk Statements

36/37/38-42

Safety Statements

22-24-26-36/37

WGK Germany

RTECS

XO8950000