T5201
Sigma

Monoclonal Anti-β-Tubulin

~2 mg/mL, clone TUB 2.1, purified immunoglobulin, buffered aqueous solution

Descriptions

Immunogen

tubulin from rat brain.

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5).¹ It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

TUB 2.1

form

buffered aqueous solution

mol wt

antigen mol wt 55 kDa

species reactivity

chicken, human, bovine, rabbit, mouse, rat, hamster, frog, moth, wheat, sea urchin, plant

concentration

~2 mg/mL

application(s)

immunoblotting²: 2-4 μg/mL using chicken fibroblasts cell extract

immunoelectrophoresis¹: suitable

immunohistochemistry³: suitable

indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Tubb1(104068), Tubb2a(22151), Tubb2c(227613)

rat ... Tubb2(29212), Tubb2c(296554)

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)

shipped in

dry ice

storage temp.

−20°C

References

Cited Reference

1. Wolff, A., et al. Biol. Cell 63, 319-326, (1988)

2. Schomai, A., and Shaul, Y. Hepatology 37, 764-770, (2003) abstract

3. Binarova, P., et al. Plant Cell 12, 433-442, (2000)

Reference

Gozes, I., et al., Monoclonal antibodies that recognize discrete forms of tubulin. Proc. Natl. Acad. Sci. USA 79, 2579, (1982)

Matthes, T., et al., Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule. J. Immunol. 141, 3135, (1988)

Gingras, D., et al. J. Biol. Chem. 271, 12807-12813, (1996)

Soroka, C.J., et al. J. Biol. Chem. 274, 26416-26424, (1999)

Safety

WGK Germany