Monoclonal Anti-β-Tubulin antibody produced in mouse 

sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.

ascites fluid

2-28-33

15 mM sodium azide

Caenorhabditis elegans, Strongylocentrotus purpuratus, Ciona intestinalis, chicken, human, bovine, rat

immunoblotting: suitable

immunocytochemistry: suitable

indirect immunofluorescence: 1:1,000 using cultured human fibroblasts

IgG1

rat ... Tubb2(29212), Tubb2c(296554)

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)

dry ice

−20°C

Siddiqui, S.S., et al. J. Neurosci. 9, 2963, (1989) abstract

Crowther, R.J., and Whittaker, J.R. J. Neurobiol. 23, 280, (1992) abstract

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