Analysis Note
Single band by SDS-PAGE.
Preparation Note
Prepared by a modification of the method of Haque, et al.
Reconstitution
When reconstituted with 100 μl of sterile water the concentration is 1 μg/μl in 40 mM sodium phosphate buffer, pH 7.2, containing 200 mM NaCl.
Biochem/physiol Actions
Cholesterol-binding toxin used to permeabilize cellular membranes to enhance the entry of macromolecules into the interior of the cell. Pores induced reported to be in the range of 20-50 nm.
General description
Hemolytic activity of tetanolysin is determined using 2.5% rabbit red blood cells at 37 °C for 40 min.
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