T5567
Sigma

Monoclonal Anti-TCF-1 (T-Cell Factor-1) antibody produced in mouse

~1 mg/mL, clone 7H3, purified immunoglobulin, buffered aqueous glycerol solution

MDL number

MFCD02686647

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Descriptions

Immunogen

MBP fusion protein corresponding to amino acids 109-269 of human TCF-1A splice variant.

Physical form

Solution in 0.1 M tris-glycine, pH 7.4, containing 0.15 M sodium chloride, 30% glycerol and 0.08% sodium azide.

Properties

antibody form

purified immunoglobulin

clone

7H3

form

buffered aqueous glycerol solution

mol wt

antigen mol wt 26-48 kDa (32, 38, 42)

species reactivity

human

concentration

~1 mg/mL

application(s)

immunoblotting: 0.5-2 μg/mL using whole extracts of Jurkat cells

immunohistochemistry (frozen sections): suitable

immunoprecipitation: suitable

isotype

IgG

Gene Information

human ... HNF1A(6927)

shipped in

wet ice

storage temp.

−20°C

References

Reference

Castrop, K., et al., The human Tcf-1 gene encodes a nuclear DNA-binding protein uniquely expressed in normal and neoplastic T-linkage lymphocytes. Blood 86, 3050, (1995) abstract

Staal, F.J., et al., Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells. Int. Immunol. 11, 317, (1999) abstract

Mayer, K., et al., Ectopic activation of lymphoid high mobility group-box transcription factor Tcf-1 and overexpression in colorectal cancer cells. Int. J. Cancer 72, 625, (1997) abstract

Safety

WGK Germany