T5817
Sigma

Monoclonal Anti-TCF-4 (T-Cell Factor-4) antibody produced in mouse

~1 mg/mL, clone 6H5-3, purified immunoglobulin, buffered aqueous glycerol solution

MDL number

MFCD02097475

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Descriptions

Application

May be used for gel-shift assays

Immunogen

His-tagged fusion protein corresponding to amino acids 31-331 of human TCF-4

Physical form

Solution in 0.1 M tris-glycine, pH 7.4, containing 0.15 M sodium chloride, 30% glycerol and 0.08% sodium azide.

Properties

antibody form

purified immunoglobulin

clone

6H5-3

form

buffered aqueous glycerol solution

mol wt

antigen mol wt 66 kDa

species reactivity

human, mouse

concentration

~1 mg/mL

application(s)

immunoblotting: 1-4 μg/mL using whole extracts of Cos-1 cells transfected with human TCF-4

immunohistochemistry: suitable using 4% formaldehyde, paraffin-embedded human and mouse tissue

immunoprecipitation: suitable

isotype

IgG2a

Gene Information

mouse ... Tcf4(21413)

human ... TCF4(6925)

shipped in

wet ice

storage temp.

−20°C

References

Reference

Barker, N., et al., Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium. Am. J. Pathol. 154, 29, (1999) abstract

Korinek, V., et al., Depletion of epithelial stem-cell compartments in the small intestine of mice lacking Tcf-4. Nat. Genet. 19, 379, (1998) abstract

Ishitani, T., et al., The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature 399, 798, (1999) abstract

Safety

WGK Germany