T6199
Sigma

Monoclonal Anti-α-Tubulin antibody produced in mouse

~1 mg/mL, clone DM1A, purified immunoglobulin, buffered aqueous solution

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Synonym

Monoclonal Anti-α-Tubulin

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Descriptions

Immunogen

microtubules from chicken embryo brain.

Specificity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

DM1A

form

buffered aqueous solution

mol wt

antigen mol wt ~50 kDa

species reactivity

chicken, human, bovine, rat, mouse, amphibian, yeast, fungi

concentration

~1 mg/mL

application(s)

immunoblotting: 0.5-1 μg/mL using total tissue extract from chicken gizzard

immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB)

immunohistochemistry: suitable

immunoprecipitation: suitable

microarray: suitable

isotype

IgG1

Gene Information

mouse ... Tuba1a(22142)

rat ... Tuba1(64158)

human ... TUBA4A(7277)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Blose, S.H., et al., 10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin. J. Cell Biol. 98, 847, (1984)

Safety

Safety Statements

23-24/25

WGK Germany