T9281
Sigma

N,N,N′,N′-Tetramethylethylenediamine

for electrophoresis, ~99%

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Synonym

1,2-Bis(dimethylamino)ethane

TEMED

TMEDA

Molecular Formula

(CH₃)₂NCH₂CH₂N(CH₃)₂

Molecular Weight

116.20

CAS Number

110-18-9

Beilstein Registry Number

1732991

MDL number

MFCD00008335

PubChem Substance ID

24900591

EG/EC Number

203-744-6

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Descriptions

Analysis Note

Tested as a catalyst for PAGE.

Application

TEMED is a tertiary amine base used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules.

Properties

grade

for electrophoresis

assay

~99%

expl. lim.

9.08 %

refractive index

n20/D 1.4179(lit.)

120-122 °C(lit.)

−55 °C(lit.)

density

0.775 g/mL at 20 °C(lit.)

References

Reference

Andrews, A.T. Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications 2nd ed., New York , (1988), 21-24

Ogden, R.C. and Adams, D.A., Electrophoresis in agarose and acrylamide gels. Meth. Enzymol. 152, 61-87, (1987) abstract

Beilstein

Beil. 4,IV,1172

Fieser

Fieser 2,403 / Fieser 3,284 / Fieser 4,485 / Fieser 5,652 / Fieser 6,576 / Fieser 7,358 / Fieser 12,477 / Fieser 13,57

reference

Aldrich MSDS 1, 1682:A / Corp MSDS 1 (2), 3296:B / FT-IR 2 (1), 478:C / FT-IR 1 (1), 305:A / FT-NMR 1 (1), 492:B / IR-Spectra (3), 178:B / IR-Spectra (2), 160:H / NMR-Reference 2 (1), 262:D / RegBook 1 (1), 327:D / Sax 6, 2545 / Sigma FT-IR 1 (2), 914:D / Structure Index 1, 45:D:1 / Vapor Phase 3, 391:A

Safety

Hazard Codes

F,C

Risk Statements

11-20/22-34

Safety Statements

16-26-36/37/39-45

RIDADR

UN2372 3/PG 2

WGK Germany

RTECS

KV7175000