TOX4
Sigma

In Vitro Toxicology Assay Kit, Neutral Red based

Descriptions

Application

For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.

Biochem/physiol Actions

Neutral red is taken up by viable cells and stored in the lysosomes. The dye is extracted and the uptake is quantitated by spectroscopy.

Properties

usage

1 kit sufficient for 1,000 tests

storage temp.

2-8°C

References

Reference

Babich, H., et al., In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida). Ecotox. Environ. Safety 21, 327-336, (1991)

Fautz, R., et al., Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: rapid colorimetric viability determination for the unscheduled DNA synthesis test (UDS). Mutat. Res. 253, 173-179, (1991) abstract

Borenfreund, E., and Puerner, J., Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24, 119-124, (1985) abstract

Morgan, C., et al., An improved colorimetric assay for tumor necrosis factor using WEHI 164 cells cultured on novel microtiter plates. J. Immunol. Methods 145, 259-262, (1991) abstract

Triglia, D., et al, In vitro toxicity of various classes of test agents using the neural red assay on a human three-imensional physiologic skin model. In Vitro Cell. Dev. Biol. 27A, 239, (1991) abstract

Safety

Hazard Codes

Risk Statements

10-36/37/38-43

Safety Statements

26-36

RIDADR

UN 3316 9/PG 3