Urea 

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

ReagentPlus is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

ReagentPlus^®

≥99.5%

pellets

132-135 °C(lit.)

H₂O: 8 M at 20 °C

1.335 g/mL at 25 °C(lit.)

human ... CA1(759), CA2(760)

rat ... Ppm1a(24666)

Mukhopadhyay, A., Inclusion bodies and purification of proteins in biologically active forms. Adv. Biochem. Eng. Biotechnol. 56, 61-109., (1997) abstract

Rudloph, R. and Lilie, H., In vitro folding of inclusion body proteins. FASEB J. 10, 49-56, (1996) abstract

Marston, F.A.O. and Hartley, D.L, Solubilization of protein aggregates. Meth. Enzymol. 182, 264-276, (1990) abstract

Merck 13,9935

Beil. 3,IV,94

Fieser 1,1262 / Fieser 2,455

Corp MSDS 1 (2), 3578:A / FT-IR 2 (1), 1356:D / FT-IR 1 (1), 799:C / IR-Spectra (3), 474:B / IR-Spectra (2), 420:F / NMR-Reference 2 (1), 670:B / RegBook 1 (1), 939:B / Sax 6, 2713 / Structure Index 1, 146:A:1

1

YR6250000