Urea 

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

for electrophoresis

≤0.005% Ammonia

132-135 °C(lit.)

1.335 g/mL at 25 °C(lit.)

Fe: ≤1 ppm

heavy metals (as Pb): ≤5 ppm

A5M/260 ≤0.05

A5M/280 ≤0.05

human ... CA1(759), CA2(760)

rat ... Ppm1a(24666)

O'Farrell, P.H., High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007, (1975) abstract

Andrews, A.T. Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications 2nd ed., New York , (1988), 252

Hochstrasser, D.F., Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173, 424, (1988) abstract

Merck 13,9935

Beil. 3,IV,94

Fieser 1,1262 / Fieser 2,455

Corp MSDS 1 (2), 3578:A / FT-IR 2 (1), 1356:D / FT-IR 1 (1), 799:C / IR-Spectra (3), 474:B / IR-Spectra (2), 420:F / NMR-Reference 2 (1), 670:B / RegBook 1 (1), 939:B / Sax 6, 2713 / Structure Index 1, 146:A:1

1

YR6250000