U8379
Sigma

UDP-Glucuronosyltransferase 1A3 Isozyme human

recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution, ≥0.04 units/mg protein

Synonym

UDP-glycosyltransferase1A3

UGT1A3

Enzyme Commission (EC) Number

2.4.1.17 ( BRENDA | IUBMB )

MDL number

MFCD03458917

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Descriptions

General description

UGT1A3 catalyzes the glucuronidation of carboxylic acid-containing drugs.

Unit Definition

One unit will transfer one nanomole of glucuronic acid from uridine-5'-diphosphoglucuronic acid to octyl gallate per minute at pH 7.5 at 37 °C

Physical form

Microsomal fraction in 100 mM potassium phosphate, pH 7.4, 20% (v/v) glycerol, 0.1 mM EDTA, 1 mM DTT

Biochem/physiol Actions

The UDP-Glucuronosyltransferases (UGT) comprise a family of enzymes that detoxify and enhance the urinary excretion of a wide variety of xenobiotic and endogenous substrates by transferring glucuronic acid to sulfhydryl, hydroxyl, aromatic amino, or carboxylic acid groups.

General description

Greater than 80% of the activity was observed after the microsomes had undergone five freeze-thaw cycles.

Properties

recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous glycerol solution

concentration

2-20 units/mL protein

Gene Information

human ... UGT1A3(54659)

shipped in

dry ice

storage temp.

−70°C

References

Reference

Gall, W.E., et al., Differential glucuronidation of bile acids, androgens and estrogens by human UGT1A3 and 2B7. J. Steroid Biochem. 70, 101, (1999)

Mackenzie, P.I. et al., Polymorphisms in UDP glucuronosyltransferase genes: functional consequences and clinical relevance. Clin. Chem. Lab Med. 38, 889, (2000)

Tukey, R.H., Strassburg, C.P., Human UDP-glucuronosyltransferases: metabolism, expression, and disease. Annu. Rev. Pharmacol. Toxicol. 40, 581, (2000)

King, C.D., et al., UDP-glucuronosyltransferases. Curr. Drug Metab. 1, 143, (2000)

Bansal, S.K. and Gessner, T. Anal. Biochem. 109, 321-329, (1980)

Green, M.D., et al., Glucuronidation of amines and other xenobiotics catalyzed by expressed human UDP-glucuronosyltransferase 1A3. . Drug Metab. Dispos. 26, 507, (1998)

Matern, H., Heinemann, H., and Matern, S. Anal. Biochem. 219, 182-188, (1994)

Safety

WGK Germany