VEGF Receptor-1 (Flt-1)/Fc Chimera human 

The biological activity is measured by its ability to Inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline and 0.3 M sodium chloride containing 50 μg bovine serum albumin per 1 μg of cytokine.

VEGF receptors, class III receptor tyrosine kinases, have seven Ig like extracellular motifs and a tyrosine kinase intracellular domain split by a kinase insert sequence. VEGFR 1 was originally called fms like tyrosine kinase 1 (flt 1), indicating its homology to receptors of PDGF, M CSF, and SCF. VEGFR 1 is one of the five receptor tyrosine kinases (KDR/Flk-1, Flt-4, tie-1 and tek/tie-2) whose expression is almost exclusively restricted to endothelial cells. These receptors are likely to play central roles in vasculogenesis and angiogenesis. Recombinant VEGF R1 binds VEGF with high affinity and is a potent VEGF inhibitor. VEGFR 1 and 2 are both expressed in an endothelial cell-specific manner and are detectable in virtually all tissues in adults and embryos. Hypoxia induces endothelial cell expression of VEGFR 1 but not VEGFR 2. Monocytes express VEGFR 1 and 2 and can migrate to the site of VEGF release, but this response appears to be VEGFR 1 mediated. VEGFR 2 is also expressed in pancreatic duct cells. VEGFR 2 is involved in commitment of endothelial-cell lineages and to cell proliferation, while VEGFR 1 seems to be responsible for guiding endothelial cells into the proper spatial organization into lumen-containing vessels. VEGFR 2 is a key marker for pluripotent HSCs. Neurophilins, a group of unrelated receptors for some VEGF family members may also act as coreceptors to facilitate VEGF binding to VEGFR 2. VEGFR 3 is a specific marker for lymphatic vessels. VEGFR 3 has also been detected on some high endothelial venules, in embryonic pre-lymphatic blood vessels, some tumor blood vessels, and certain hematopoietic and leukemia cells.

expressed in Sf21 cells

>90% (SDS-PAGE)

lyophilized powder

Endotoxin, tested

human ... FLT1(2321)

−20°C

Ziegler, B.L., et al., KDR receptor: a key marker defining hematopoietic stem cells. Science 285, 1553-1558, (1999)

Shibuya, M., et al., Ikada, Y., and Okano, T., ed., Signal transduction of vascular endothelial growth factor (VEGF) receptors, Fltand KDR/Flkin Tissue Engineering for Therapeutic Use 3,. Int. Congr. Ser., (1999) 1175

Taipale, J., et al., Vascular endothelial growth factor receptor Curr. Top. Curr. Top. Microbiol. Immunol. 237, 85, (1999)

Neufeld, G., et al., Vascular endothelial growth factor (VEGF) and its receptors. FASEB J. 13, 9-22, (1999)

Shibuya, M., et al., Structure and function of vascular endothelial growth factor receptor and Curr. Top. Microbiol. Immunol. 237, 59, (1999)

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