General description
The SYBR Green Extract-N-Amp Plant PCR Kit is a complete system for the rapid extraction of genomic DNA from plant leaf tissue coupled with amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated. Kits contain all necessary reagents for both extraction and amplification.
The SYBR Green Extract-N-Amp system is reagent-based and does not require purification columns, organic solvents or caustic lysis agents. Two solutions combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
Specifications
The protocol uses a 20 μl PCR reaction.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR is a registered trademark of Molecular Probes, Inc.
SYBR is a registered trademark of Molecular Probes, Inc.
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