Components
Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.
Other Notes
Features and Benefits
• Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
• Compatible with high-throughput requirements for genetic analysis of plants
• Extract stable at 4 °C for at least 6 months (Fig. 3)
General description
The REDExtract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Purchase of these products is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.