REDExtract-N-Amp^™ Tissue PCR Kit 

Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

• Single-step extraction of genomic DNA for PCR in 15 minutes
• Perfect for quick genomic DNA isolation for genotyping
• Protocols available for mouse-tails, hair, animal tissue, saliva, and buccal swabs
• A PCR ReadyMix^™, specially formulated for amplification directly from extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA

The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient lading of PCR reactions onto agarose gels for analysis.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Extract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of these products is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

sufficient for 1000 amplifications

sufficient for 1000 extractions

wet ice

−20°C

N3910, Neutralization Solution B