SYBR^® Green Extract-N-Amp^™ Tissue PCR Kit 

Kit contains Extraction Solution, Neutralization Solution, Tissue Preparation Solution, and Extract-N-Amp SYBR Green PCR ReadyMix^™. The Extract-N-Amp SYBR Green PCR ReadyMix is a 2X reaction mix containing SYBR Green, buffer, salts, dNTPs, Taq polymerase and JumpStart™ Taq antibody. It is optimized specifically for use with the extraction reagents and contains JumpStart Taq antibody for hot start PCR to enhance specificity and SYBR Green I to act as a nonspecific reporter for real-time PCR.

The SYBR Green Extract-N-Amp Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. There is no need for freezing tissue in liquid nitrogen, mechanical disruption, organic extraction, column purification or precipitation of the DNA. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp SYBR Green PCR ReadyMix™ and user-provided PCR primers.

Extract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR® Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR® is a registered trademark of Molecular Probes, Inc.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

SYBR is a registered trademark of Molecular Probes, Inc.

sufficient for 1000 amplifications

sufficient for 1000 extractions