Yeast Transformation Kit 

Contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.¹ The lithium acetate method is documented by a long, successful publication history as a highly efficient and low cost method of transformation. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 µg range. The Yeast Transformation Kit contains all necessary reagents and is ready-to-use – no mixing is necessary. Offers complete flexibility so that any strain of yeast can be used.

• Ready-to-use
• As little as 10 ng of plasmid DNA required
• Flexibility for any strain of yeast

1 kit sufficient for >100 standard transformations

−20°C

1. Ito, H., et al., Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153, 163-168, (1983) abstract

Gietz, D., et al., Improved method for high efficiency transformation of yeast. Nucleic Acids Res. 20, 1425, (1992) abstract

Elble, R., A simple efficient procedure for transformation of yeast. Biotechniques 13, 18-20, (1992) abstract

Hill, J., et al., DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res. 19, 5791, (1991) abstract

Carrier DNA from salmon testes 2×10 mg D9156

Control yeast plasmid DNA, pRS316 10 μg

PLATE buffer 100 mL

Transformation buffer 100 mL

Yeast synthetic media supplement without uracil 1 g Y1501