| Z306207 Sigma |
DNA Cloning: A Practical Approach, 2nd ed.Volume 4: Mammalian Systems |
General description
Topics covered include: high efficiency gene transfer into mammalian cells, use of vectors based on gene amplification, retroviral vectors, genetic manipulation of embryonic stem cells, production of transgenic rodents, genomic and expression analysis of transgenic animals, expression using a defective HSV-1 vector system, and adenovirus vectors.
Table of Contents
1. High efficiency gene transfer into mammalian cells
2. Construction and characterization of Vaccinia virus recombinants
3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells
4. Retroviral vectors
5. Genetic manipulation of embryonic stem cells
6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs
7. Genomic and expression analysis of transgenic animals
8. Expression using a defective Herpes simplex virus (HSV-1) vector system
9. Adenovirus vectors
2. Construction and characterization of Vaccinia virus recombinants
3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells
4. Retroviral vectors
5. Genetic manipulation of embryonic stem cells
6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs
7. Genomic and expression analysis of transgenic animals
8. Expression using a defective Herpes simplex virus (HSV-1) vector system
9. Adenovirus vectors
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