| Z362360 Sigma |
DNA Cloning: A Practical Approach, 2nd ed.Volume 1: Core Techniques |
General description
Topics include transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and gene expression, in vitro mutagenesis, and DNA sequencing.
Table of Contents
1. Techniques for transformation of E.coli
2. Lambda replacement vectors in the construction of genomic DNA libraries
3. Procedures for cDNA cloning
4. Making probes
5. Using cloned DNAs to analyse gene organization and expression
6. Choices of single-stranded vectors and their application for in vitro mutagenesis
7. PCR and its applications
8. DNA sequencing
2. Lambda replacement vectors in the construction of genomic DNA libraries
3. Procedures for cDNA cloning
4. Making probes
5. Using cloned DNAs to analyse gene organization and expression
6. Choices of single-stranded vectors and their application for in vitro mutagenesis
7. PCR and its applications
8. DNA sequencing
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