| Z362379 Sigma |
DNA Cloning: A Practical Approach, 2nd ed.Volume 2: Expression Systems |
General description
This volume provides essential information and full details of current procedures for the expression of cDNA clones in E. coli, lambda-phage, yeast, and baculovirus expression systems. Also covered are the isolation and purification of recombinant protein(s), and the generation of monoclonal and polyclonal antibodies.
Table of Contents
1. Screening expression libraries with antibody and protein probes
2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
3. Purification of over-produced proteins from E.coli cells
4. Production of monoclonal antibodies against proteins expressed in E.coli
5. Expression of cloned genes in yeast
6. Intraction trap cloning with yeast
7. The baculovirus expression system
2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies
3. Purification of over-produced proteins from E.coli cells
4. Production of monoclonal antibodies against proteins expressed in E.coli
5. Expression of cloned genes in yeast
6. Intraction trap cloning with yeast
7. The baculovirus expression system
Related Information
Links
Page Options| |
|
© 2008 Sigma-Aldrich Co. Reproduction forbidden without permission. |