| Z379794 Sigma |
Antisense Technology: A Practical Approach |
General description
Use of antisense oligonucleotides is a powerful technique for silencing specific genes, but it requires careful optimization and species-specific methods for success. Each chapter is written by an acknowledged expert with a record of success at the bench; each includes authoritative guidance, tips for success, and trouble-shooting advice.
Table of Contents
1. Nucleic acid structures
2. Evaluation of antisense effects
3. Selecting, preparing, and handling antisense oligodeoxyribonucleotides
4. In vitro RNAs
5. Catalytic antisense RNA based on hammerhead ribozymes
6. 2-5 A-Antisense chimaeras for targeted degradation of RNA
7. In vitro applications of antisense RNA and DNA
8. Antisense applications in Dictyostelium: a lower eukaryotic model system
9. Applications of antisense technology in plants
10. Antisense molecules and ribozymes: medical applications
11. Non-antisense effects of oligodeoxynucleotides
12. Antisense rescue
13. Shotgun antisense mutants
14. Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies
2. Evaluation of antisense effects
3. Selecting, preparing, and handling antisense oligodeoxyribonucleotides
4. In vitro RNAs
5. Catalytic antisense RNA based on hammerhead ribozymes
6. 2-5 A-Antisense chimaeras for targeted degradation of RNA
7. In vitro applications of antisense RNA and DNA
8. Antisense applications in Dictyostelium: a lower eukaryotic model system
9. Applications of antisense technology in plants
10. Antisense molecules and ribozymes: medical applications
11. Non-antisense effects of oligodeoxynucleotides
12. Antisense rescue
13. Shotgun antisense mutants
14. Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies
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