| Z380709 Sigma |
ICRF Handbook of Genome Analysis |
General description
This two-volume set, “the handbook for the genome revolution,” is an essential guide to the enormous range of techniques for genetic and physical genome mapping, as well as sequencing and analysis of DNA. It is both a protocol manual and a comprehensive reference. The primary focus is on human genetics, but the book also considers other model organisms − bacterial, plant and animal. Exhaustive appendices include Internet resources, basic data on human and other genomes, and mouse gene knock-outs.
Table of Contents
Introduction; Family Studies; Complex studies; Constructing and
using genetic maps; Automated map construction; Polymorphic
Markers; DNA typing;
Section II: Physical mapping I: Cytogenetic analysis;
Introduction; Conventional cytogenetics; Solid tumor cytogenetics; In situ hybridization; Chromosome painting; Chromosome microdissection; Chromosome sorting and analysis; Digital microscopy;
Section III: Physical mapping II: Long-range mapping and gene isolation; Introduction; Somatic cell hybrid approaches to genome mapping; Long-range physical mapping; Integrating
genetic and physical maps; Strategies for rapid isolation of genes from genomic DNA:cDNA techniques; Screening cDNA libraries by transient expression in mammalian cells;
Section IV: Sequencing and sequence analysis; Introduction;
Sequencing strategies; Template amplification and purification;
Sequencing chemistries; Electrophoresis; Sequence labeling and detection; Automation; Mutation analysis; Repeat analysis;
Section V: Other model systems; Introduction; Mouse mapping;
Somatic gene therapy; Drosophila melanogaster; Caenorhabditis elegans; Saccharomyces cerevisiae; Escherichia coli; Problems in plant genome analysis; Arabidopsis thaliana;
Section VI: Internet resources; Introduction; Internet resources
and how to use them; Unix survival kit; Databases and genome
resource centers;
Appendices; Materials, media and solutions; Preparation of blood bottles and processing of blood samples; Commercial suppliers; Other resources; Useful addresses and Internet connections; Basic data on human and other genomes; Catalogue of mapped human disease genes; Mouse gene knock-out tables; Chromosome aberrations associated with cancer; Glossary; Index
using genetic maps; Automated map construction; Polymorphic
Markers; DNA typing;
Section II: Physical mapping I: Cytogenetic analysis;
Introduction; Conventional cytogenetics; Solid tumor cytogenetics; In situ hybridization; Chromosome painting; Chromosome microdissection; Chromosome sorting and analysis; Digital microscopy;
Section III: Physical mapping II: Long-range mapping and gene isolation; Introduction; Somatic cell hybrid approaches to genome mapping; Long-range physical mapping; Integrating
genetic and physical maps; Strategies for rapid isolation of genes from genomic DNA:cDNA techniques; Screening cDNA libraries by transient expression in mammalian cells;
Section IV: Sequencing and sequence analysis; Introduction;
Sequencing strategies; Template amplification and purification;
Sequencing chemistries; Electrophoresis; Sequence labeling and detection; Automation; Mutation analysis; Repeat analysis;
Section V: Other model systems; Introduction; Mouse mapping;
Somatic gene therapy; Drosophila melanogaster; Caenorhabditis elegans; Saccharomyces cerevisiae; Escherichia coli; Problems in plant genome analysis; Arabidopsis thaliana;
Section VI: Internet resources; Introduction; Internet resources
and how to use them; Unix survival kit; Databases and genome
resource centers;
Appendices; Materials, media and solutions; Preparation of blood bottles and processing of blood samples; Commercial suppliers; Other resources; Useful addresses and Internet connections; Basic data on human and other genomes; Catalogue of mapped human disease genes; Mouse gene knock-out tables; Chromosome aberrations associated with cancer; Glossary; Index
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