| Z700177 Sigma |
Genetic Library Construction and Screening |
General description
This volume is designed to provide an easily accessible introduction to core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies and specialized expression cloning and library screening strategies.
Table of Contents
Strategies for cDNA Cloning and Mapping RNA Transcripts.-
Cloning PCR Products.-
Site Directed Deletion, Insertion, and Substitution Using PCR.-
Long RT-PCR Cloning -
Amplification of Full-Length Enterovirus Genomes.-
Construction of cDNA Libraries from Small Quantities of Total RNA.-
Subtractive Hybridization and cDNA Cloning.-
Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.-
Two Hybrid cDNA Cloning.-
Yeast One and Two Hybrid cDNA Cloning.-
High-Throughput Library Screening by Fluorescent Hybridizations on Gridded Membranes.-
Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries
Cloning PCR Products.-
Site Directed Deletion, Insertion, and Substitution Using PCR.-
Long RT-PCR Cloning -
Amplification of Full-Length Enterovirus Genomes.-
Construction of cDNA Libraries from Small Quantities of Total RNA.-
Subtractive Hybridization and cDNA Cloning.-
Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.-
Two Hybrid cDNA Cloning.-
Yeast One and Two Hybrid cDNA Cloning.-
High-Throughput Library Screening by Fluorescent Hybridizations on Gridded Membranes.-
Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries
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