| Z700452 Sigma |
Guide to Yeast Genetics and Molecular and Cell Biology, Part B, Methods in Enzymology Vol 350 |
General description
This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable anyone to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.
Table of Contents
PARTIAL
Section I. Basic Techniques
1. Getting Started with Yeast
2. How to Set up a Yeast Laboratory
3. Constructing Yeast Libraries
4. Transformation of Yeast by Lithium Acetate/ Single -Stranded Carrier DNA/ Polyethylene Glycol Method
5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria
6. LacZ Assays in Yeast
7. Analysis of Budding Patterns
8. Uses and Abuses of HO Endonuclease
9. Assays for Gene Silencing in Yeast
Section II. Making Mutants
10. Classical Mutagenesis Techniques
11. Introduction of Point Mutations into Cloned Genes
12. Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast
13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro
14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae
15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Meidated PCR
Section III. Genomics
19. Saccharomyces Genome Database
20. Three Yeast Proteome Databases: YPD, PombePD, and CalPD (MycoPathPD)
Section IV. Proteomics
28. Array-Based Methods for Identifying Protein-Protein and Protein-Nucleic Acid Interactions
29. Building Protein-Protein Networks by Two-Hybrid Maring Strategy
Section I. Basic Techniques
1. Getting Started with Yeast
2. How to Set up a Yeast Laboratory
3. Constructing Yeast Libraries
4. Transformation of Yeast by Lithium Acetate/ Single -Stranded Carrier DNA/ Polyethylene Glycol Method
5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria
6. LacZ Assays in Yeast
7. Analysis of Budding Patterns
8. Uses and Abuses of HO Endonuclease
9. Assays for Gene Silencing in Yeast
Section II. Making Mutants
10. Classical Mutagenesis Techniques
11. Introduction of Point Mutations into Cloned Genes
12. Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast
13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro
14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae
15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Meidated PCR
Section III. Genomics
19. Saccharomyces Genome Database
20. Three Yeast Proteome Databases: YPD, PombePD, and CalPD (MycoPathPD)
Section IV. Proteomics
28. Array-Based Methods for Identifying Protein-Protein and Protein-Nucleic Acid Interactions
29. Building Protein-Protein Networks by Two-Hybrid Maring Strategy
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