| Z700460 Sigma |
Guide to Yeast Genetics and Molecular and Cell Biology, Part C, Methods in Enzymology Vol 351 |
General description
This volume and its companion, Volume 350, are specifically designed to meet the needs of researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable anyone to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.
Table of Contents
PARTIAL
Section I. Cytology
1. Digital Teim-Lapse Microscopy of Yeast Cell Growth
2. Quantitative Microscopy of Green Fluorescent Protein-Labeled Yeast
3. Fluorescence Resonance Energy Transfer Using Color Variants of Green Fluorescent Protein
4. Immunoelectron Microscopy of Aldehyde-Fixed Yeast Cells
5. Electron Tomography of Yeast Cells
6. Cryomethods for Thin Section Electron Microscopy
Section II. Biochemistry
7. Vacuolar Proteases and Proteolytic Artifacts in Saccharomyces cerevisiae
8. Analysis of the SIze and Shape of Protein Complexes from Yeast
9. Purification of Glutathuione S-Transferase Fusion Proteins from Yeast
10. Protein- and Immunoaffinity Purification of Multiprotein Complexes
Section III. Cell Fractionation
18. Subcellular Fractionation of Secretory Organelles
19. Plasma Membrane Biogenesis
Section IV. Cell Biology
27. Synchronization Procedures
28. Separation of Mother and Daughter Cells
Section I. Cytology
1. Digital Teim-Lapse Microscopy of Yeast Cell Growth
2. Quantitative Microscopy of Green Fluorescent Protein-Labeled Yeast
3. Fluorescence Resonance Energy Transfer Using Color Variants of Green Fluorescent Protein
4. Immunoelectron Microscopy of Aldehyde-Fixed Yeast Cells
5. Electron Tomography of Yeast Cells
6. Cryomethods for Thin Section Electron Microscopy
Section II. Biochemistry
7. Vacuolar Proteases and Proteolytic Artifacts in Saccharomyces cerevisiae
8. Analysis of the SIze and Shape of Protein Complexes from Yeast
9. Purification of Glutathuione S-Transferase Fusion Proteins from Yeast
10. Protein- and Immunoaffinity Purification of Multiprotein Complexes
Section III. Cell Fractionation
18. Subcellular Fractionation of Secretory Organelles
19. Plasma Membrane Biogenesis
Section IV. Cell Biology
27. Synchronization Procedures
28. Separation of Mother and Daughter Cells
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