General description
The manufacture, experimental design, and analysis of microarrays are not always straightforward, and researchers run into roadblocks that hinder progress. This book is directed to the core facility manager who produces, hybridizes, and scans arrays, and the research scientist who will be performing the analysis and interpreting the results. It provides a user friendly coverage and protocols for the technical steps and procedures involved in many facets of microarray technology, including: Coating glass slides; Designing oligonucleotide probes; Troubleshooting spotting problems; Normalizing background signal and controlling for systematic variance; Analyzing data with statistical procedures.