FISH, A Practical Approach 

This laboratory manual covers the theory and application of the commonly used FISH procedures. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labeling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics.