Membrane Protein Protocols: Expression, Purification, and Characterization 

1-58829-124-3

978-1-58829-124-0

B. Selinsky, ed., Humana Press, 2003, 330 pp., hard cover

Part I. Expression of Membrane Proteins
1. Expression and Purification of the Amphipathic Form of Rabbit Cytochrome b5 in Escherichia coli
2. Dihydroorotate Dehydorgenase of Escherichia coli
3. A General Approach for Heterologous Membrane Protein Expresiion in Escherichia coli: The Uncoupling Protein, UCP1, as an Example
4. Expression of Membrane-Bound Iron-Sulfur Proteins
5. Heterologous Expression of Human Scavenger Receptro Calss B Type I (SR-BI) in Pichia pastoris
6. Expression of Oligomeric Amiloride-Sensitive Epithelial Sodium Channel in Sf9 Insect Cells
7. Functional Expression of His-tagged Rhodopsin Sf9 Insect Cells
Part II Detergent Selection in Membrane Protein Purification
8. Preparation of Glycerol Facilitator for Protein Strucutre and Folding Studies in Solution
9. Solubilization of Chemokine Receptors from Cell Membranes
10. A Systematic Approach for the Solubilzation of the Integral Membrane Protein Lysophospholipid: Acyl-CoA
Part III. Membrane Protein Purification and Reconstitution
11. Purification of Omp50, a Minor Porin of Campylobacter jejuni
12. Purification of Porins from Mycobacterium smegmatis
13. Isolation of the Melanocortin 5 Receptor: From cDNA Sequence to Isolating an Integral Membrane Protein
14. Purifcation of Mammalian Serine Palmitoyltransferase, a Hetero-Subunit Enzyme for Sphingolipid Biosynthesis by Affinity-Peptide Chromatography
15. Purification of Phosphatidylglycerophosphage Synthase form Cultured Mammalian Cells
16. Purification of Pancreastatin Receptor from Rat Liver Membranes
17. Purification, Reconstitution, and Functional Characterization of Zinc Transporter from Rat Renal Brush Border Membranes
18. Isolatiuon of Lipid Raft-Associated Proteolipids
19. Purification of Membrane-Bound Catechol-O-Methyltransferase
20. Purification and Characterization of Transporter Proteins from Human Erythrocyte Membrand
21. Purification of the Human Erythrocyte PS-Stimulated Mg2+-ATPase: A Putative PS Flippase
22. Reconstitution and Assay of Biogenic Membrane-Derived Phospholipid Flippase Activity in Proteoliposomes
23. Expression, Purification, and Reconstitution of Rat Liver Carnitine Palmitoyltransferase I
Part IV. Structural Analysis of Membrane Proteins
24. Crystallization in Lipidic Cubic Phases: A Case Study with Bacteriorhodopsin
25. Optical Biosensor Assay Using Retroviral Receptor Pseudotypes
Index