Cancer Cell Signaling: Methods and Protocols 

1-58829-075-1

978-1-58829-075-5

D. Terrian, ed., Humana Press, 2003, 352 pp., hard cover

Part I. Manipulation and Detection of Survival Signals
1. Functional Analysis of the Antimitogenic Activity of Tumor Suppressors
2. Rescue and Isolation of Rb-deficient Prostate Epithlium by Tissue Recombination
3. Signal Transduction Study Using Gene Targeted Embryonic Stem Cells
4. The Use of the Yeast Two Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress
5. Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
6. Mitogen Activated Protein Kinase Signaling in Drug Resistant Neuroblastoma Cells
7. TUNEL and Immunofluorescence Double Labeling Assay for Apoptotic Cells with Specific Antigen(s)
Part II. Manipulation and Detection of Oncogenic Signals
8. Kinetworks (TM) Protein Kinase Multiblot Analysis
9. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers
10. Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay
11. Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/ In Situ Assay
12. Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells
13. Measurements of Phospholipases A2, C, and D (PLA2, PLC and PLD): In Vitro Microassays, Analysis of Enzyme Isoforms, and Intact Cell Assays
14. Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model
15. Fibroblastic, Hematopoietic and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer
16. Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes
17. Eludication of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes
Part III. Protein Interactions
18. Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology
19. Production of Ligand-Specific Mutants Using a Yeast Two Hybrid Mating Assay
20. Coimmunoprecipitation Assay for the Detection of Kinase Substrate Interactions
Part IV. Genomic Rearrangements
21. Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing
22. Clonality Analysis by T-Cell Receptor PCR and High Resolution Electrophoresis in the Diagnosis of Cutaneous Lymphoma (CTCL)