HPLC of Peptides and Proteins: Methods and Protocols 

0-89603977-3

978-0-89603977-3

M. Aguilar, ed., Humana Press, 2004, 428 pp., hard cover

PART I MODES OF HPLC
1. HPLC of peptides and Proteins: Basic Theory and Methodology
2. Reversed-Phase High-Performance Liquid Chromatography
3. Ion-exchange Chromatography
4. High-Performance Hydrophobic Ínteraction Chromatography
5. Gel Filtration Chromatography
6. Hydrophilic Interaction Chromatography
7. Immobilized Metal Ion Affinity Chromatography of Proteins
8. Immunoaffinity Chromatography of Proteins
9. Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins
PART II PREPARATIVE METHODOLOGIES
10. Capillary Separations
11. Micropreparative HPLC of Peptides and Proteins
12. Multidimensional HPLC Purification of Proteins
13. Analytical High-Performance Liquid Chromatography
14. Prep/Semiprep Separations of Proteins
15. Large-Scale Protein Chromatography
PART III APPLICATIONS
16. Proteolytic Peptide Mapping
17. Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation
18. Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Sprectrometry and Liquid Chromatography-Tandem Mass Spectrometry
19. HPLC in the Analysis of Peptide Metabolism
20. Isolation and Characterization of Natrually Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells
21. Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques
22. HPLC and Mass Spectrometry of intrinsic Membrane Proteins
23. IgG Purification
24. DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions
25. Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC
26. Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry
27. Proteome Analysis
Index