Z702242
Sigma

Isolation and Purification of Proteins

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General description

This book discusses rational design of downstream protein processing; product release from cells and tissues by mechanical disruption, chemical agency and enzymatic lysis; genetic strategies to facilitate purification of recombinant proteins; solid-liquid spearation via centrifugation, flocculation, filtration and filter aids; the usage of membrane filtration for protein isolation; nonselective and selective precipitation; protein crystallization on a large scale; extraction in aqueous two-phase systems and expanded bed absorption for rapid protein isolation; robust, superporous chromatography matrices for in crude feeds, and more.

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Identifiers

ISBN-10

0-82470726-5

ISBN-13

978-0-82470726-2

Properties

publication info

R. Hatti-Kaul and B Mattiasson, ed., Marcel Dekker, 2003, 688 pp., hard cover

Table of Contents

Release of Protein from Biological Host
Chromatography: The High Resolution Technique for Protein Separation
Selectivity in Affinity Chromatography
Genetic Approaches to Facilitate Purification
Solid-Liquid Separation
Membranes for Protein Isolation and Purification
Precipitation of Proteins–Non-Specific and Specific
Protein Crystallization for Large-Scale Bioseparation
Extraction for Rapid Protein Isolation
Adsorption as an Initial Step for the Capture of Proteins
Fast Chromatography of Proteins Novel Approaches to the Chromatography of Proteins
Scale Up and Commercial Production of Recombinant Proteins
Process Monitoring in Downstream Processing
Protein Formulation
Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purification

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Z702242 (Sigma)