General description
With step-by-step protocols, this edition presents up-to-date molecular and cellular methods and techniques, including additional methods for analysis of DNA-protein interactions by DNA footprinting and gel retardation assay, mRNA differential display, and microarray technology; Explains the principles behind each method; Includes helpful trouble-shooting guides. This edition includes: new protocols in all chapters, including alternative protocols; In vitro transcription methods; analysis of DNA sequences; new bioseparation techniques. New chapters cover: mRNA differential display; Inhibition of gene expression; In situ hybridization; Combinatorial techniques; Computational data mining methods applied to combinatorial chemistry libraries.