PCR/RT-PCR in situ Light and Electron Microscopy 

Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. This book covers methods of in situ PCR and reverse transcription PCR (RT-PCR), two approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. It provides theoretical consideration, as well as practical approaches to in situ PCR. Authors provide detailed protocols including the preparation of tissue samples, the rationale for the design of primers and revelation. They emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity.