Bio conjugation Protocols: Strategies and Methods 

1-58829098-0

978-1-58829098-4

C. Niemeyer, Humana Press, 2004, 344 pp., hard cover

PART I ANTIBODY AND ENZYME CONJUGATES
1. Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress
2. Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals
3. Smart Polymer-Streptavidin Conjugates
4. Conjugates of Peptides and Proteins to Polyethylene Glycols
5. Chemical Production of Bispecific Antibodies
6. Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties
7. Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase
PART II NUCLEIC ACID CONJUGATES
8. Fluorescent Sample Labeling for DNA Microarray Analyses
9. High-Density Labeling of DNA for Single Molecule Sequencing
10. Sequence-Specific DNA Labeling Using Methyltransferases
11. Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications
12. Covalent Coupling of DNA Oligonucleotides and Streptavidin
13. Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates
14. Synthesis of Peptide Nucleic Acid-Peptide Conjugates
PART III GLYCOSYL AND LIPID CONJUGATES
15. Protein Lipidation
16. Synthesis of Lipidated Peptides
17. In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins
18. Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling
19. Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2
20. Subtilisin-Catalyzed Glycopeptide Condensation
PART IV BIOFUNCTIONALIZATION OF SURFACES
21. Peptide Nucleic Acid Microarrays
22. Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles
23. Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging
Index